other:Article Title: Knock-out and pull-out recombineering protocols for naturally transformable Burkholderia thailandensis and Burkholderia pseudomallei
Article Snippet: L9330 and L9310) LS agar (LB agar without NaCl; e.g. Teknova, cat. no. L9200) Saccharides: L-arabinose (2 M filter sterilized; e.g., MP Biomedicals, cat. no. 10076); L-rhamnose (10% (w/v) filter sterilized; e.g., Sigma-Aldrich, cat. no. R3875); D-glucose (1 M filter sterilized; e.g., Sigma-Aldrich, cat. no. G7528); Sucrose (1 M filter sterilized; e.g., Tekanova, cat. no. S0002) DL-chlorinated phenylalanine (cPhe; e.g., Acros Organics, cat. no. 15728-0050) Enzyme: Pfu polymerase (e.g., Stratagene, cat. no. 600153) CaCl 2 (1 M, autoclaved in water; e.g., Acros Organics, cat. no. 194635) MgSO 4 (1 M, autoclaved in water; e.g. Acros Organics, cat. no. 194699) M9 minimal salts (5×, e.g., Sigma-Aldrich, cat. no. M6030); alternative preparation 19 Agar (molecular biology grade; e.g., Teknova cat. no. A777) Agarose (molecular grade; e.g. Research Products International, cat. no. A20065-100.0) Glyphosate, super-concentrated Round Up ® (50% (v/v) GS from Home Depot or other farm/garden supply stores)
Article Title: Knockout and pullout recombineering for naturally transformable Burkholderia thailandensis and Burkholderia pseudomallei
Article Snippet: L9330 and L9310) LS agar (LB agar without NaCl; Teknova, cat. no. L9200) Saccharides: l-arabinose (2 M; filter sterilized; MP Biomedicals, cat. no. 10076); l-rhamnose (10% (wt/vol) filter sterilized; Sigma-Aldrich, cat. no. R3875); d-glucose (1 M; filter sterilized; Sigma-Aldrich, cat. no. G7528); sucrose (1 M; filter sterilized; Tekanova, cat. no. S0002) dl-chlorinated phenylalanine (cPhe; Acros Organics, cat. no. 15728-0050) Enzyme Pfu polymerase (Stratagene, cat. no. 600153) CaCl 2 (1 M; autoclaved in water; Acros Organics, cat. no. 194635) MgSO 4 (1 M; autoclaved in water; Acros Organics, cat. no. 194699) M9 minimal salts (5×; Sigma-Aldrich, cat. no. M6030); alternative preparation19 Agar (molecular biology grade; Teknova, cat. no. A777) Agarose (molecular grade; Research Products International, cat. no. A20065-100.0) TAE buffer (20×; Thermo Scientific Pierce Protein Research Products, cat. no. 28354); alternative preparation19 • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Glyphosate: super-concentrated RoundUp (50% (vol/vol) GS from Home Depot or other farm or garden supply stores) Kanamycin sulfate salt (Sigma-Aldrich, cat. no. K4378)
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